• Western blot analysis of extracts of various cell lines, using BUB1B antibody (STJ116736) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-BUB1B antibody (160-400) (STJ116736)

SKU:
STJ116736

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BUB1B (160-400) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BUB1B
Gene ID: 701
Uniprot ID: BUB1B_HUMAN
Immunogen Region: 160-400
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 160-400 of human BUB1B (NP_001202.4).
Immunogen Sequence: EEYEARENFRKADAIFQEGI QQKAEPLERLQSQHRQFQAR VSRQTLLALEKEEEEEVFES SVPQRSTLAELKSKGKKTAR APIIRVGGALKAPSQNRGLQ NPFPQQMQNNSRITVFDENA DEASTAELSKPTVQPWIAPP MPRAKENELQAGPWNTGRSL EHRPRGNTASLIAVPAVLPS FTPYVEETAQQPVMTPCKIE PSINHILSTRKPGKEEGDPL QRVQSHQQASEEKKEKMMY
Tissue Specificity Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index.
Post Translational Modifications Proteolytically cleaved by caspase-3 in a cell cycle specific manner. The cleavage might be involved in the durability of the cell cycle delay. Caspase-3 cleavage is associated with abrogation of the mitotic checkpoint. The major site of cleavage is at Asp-610. Acetylation at Lys-250 regulates its degradation and timing in anaphase entry. Ubiquitinated. Degraded by the proteasome. Sumoylated with SUMO2 and SUMO3. The sumoylation mediates the association with CENPE at the kinetochore. Autophosphorylated in vitro. Intramolecular autophosphorylation is stimulated by CENPE. Phosphorylated during mitosis and hyperphosphorylated in mitotically arrested cells. Phosphorylation at Ser-670 and Ser-1043 occurs at kinetochores upon mitotic entry with dephosphorylation at the onset of anaphase.
Function Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.
Protein Name Mitotic Checkpoint Serine/Threonine-Protein Kinase Bub1 Beta
Mad3/Bub1-Related Protein Kinase
Hbubr1
Mitotic Checkpoint Kinase Mad3l
Protein Ssk1
Database Links Reactome: R-HSA-141430
Reactome: R-HSA-141444
Reactome: R-HSA-174184
Reactome: R-HSA-176409
Reactome: R-HSA-179409
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-5663220
Reactome: R-HSA-68877
Reactome: R-HSA-9648025
Cellular Localisation Cytoplasm
Nucleus
Chromosome
Centromere
Kinetochore
Cytoskeleton
Microtubule Organizing Center
Centrosome
Cytoplasmic In Interphase Cells
Associates With The Kinetochores In Early Prophase
Kinetochore Localization Requires Bub1
Plk1 And Knl1
Alternative Antibody Names Anti-Mitotic Checkpoint Serine/Threonine-Protein Kinase Bub1 Beta antibody
Anti-Mad3/Bub1-Related Protein Kinase antibody
Anti-Hbubr1 antibody
Anti-Mitotic Checkpoint Kinase Mad3l antibody
Anti-Protein Ssk1 antibody
Anti-BUB1B antibody
Anti-BUBR1 antibody
Anti-MAD3L antibody
Anti-SSK1 antibody

Information sourced from Uniprot.org

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