• Western blot analysis of extracts of various cell lines, using BNIP3 antibody (STJ11101717) at 1:1000 dilution. Hela cells and NIH/3T3 cells were treated by Cobalt chloride (0. 1 mM) at 37 °C for 4 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded human liver using BNIP3 rabbit monoclonal antibody (STJ11101717) at dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat heart using BNIP3 rabbit monoclonal antibody (STJ11101717) at dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using BNIP3 rabbit monoclonal antibody (STJ11101717) at dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-BNIP3 antibody (40-120) [S7MR] (STJ11101717)

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STJ11101717

$197.78 - $863.33
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-BNIP3 (40-120) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BNIP3
Gene ID: 664
Uniprot ID: BNIP3_HUMAN
Immunogen Region: 40-120
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 40-120 of human BNIP3 (NP_004043.3).
Immunogen Sequence: AFPPPAAEAHPAARRGLRSP QLPSGAMSQNGAPGMQEESL QGSWVELHFSNNGNGGSVPA SVSIYNGDMEKILLDAQHES G
Function Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway.
Protein Name Bcl2/Adenovirus E1b 19 Kda Protein-Interacting Protein 3
Cellular Localisation Mitochondrion
Mitochondrion Outer Membrane
Single-Pass Membrane Protein
Coexpression With The Eib 19-Kda Protein Results In A Shift In Nip3 Localization Pattern To The Nuclear Envelope
Colocalizes With Acaa2 In The Mitochondria
Colocalizes With Spata18 At The Mitochondrion Outer Membrane
Alternative Antibody Names Anti-Bcl2/Adenovirus E1b 19 Kda Protein-Interacting Protein 3 antibody
Anti-BNIP3 antibody
Anti-NIP3 antibody

Information sourced from Uniprot.org

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