• Western blot analysis of extracts of various cell lines, using BMF antibody (STJ28360) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.

Anti-BMF antibody (1-184) (STJ28360)

SKU:
STJ28360

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BMF (1-184) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BMF
Gene ID: 90427
Uniprot ID: BMF_HUMAN
Immunogen Region: 1-184
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-184 of human BMF (NP_277038.1).
Immunogen Sequence: MEPSQCVEELEDDVFQPEDG EPVTQPGSLLSADLFAQSLL DCPLSRLQLFPLTHCCGPGL RPTSQEDKATQTLSPASPSQ GVMLPCGVTEEPQRLFYGNA GYRLPLPASFPAVLPIGEQP PEGQWQHQAEVQIARKLQCI ADQFHRLHVQQHQQNQNRVW WQILLFLHNLALNGEENRNG AGPR
Tissue Specificity Isoform 1 is mainly expressed in B-lymphoid cells. Isoform 2 and isoform 3 are mainly expressed in B-CLL and normal B-cells.
Function May play a role in apoptosis. Isoform 1 seems to be the main initiator.
Protein Name Bcl-2-Modifying Factor
Database Links Reactome: R-HSA-111453
Reactome: R-HSA-139910
Alternative Antibody Names Anti-Bcl-2-Modifying Factor antibody
Anti-BMF antibody

Information sourced from Uniprot.org

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