• Immunofluorescence analysis of U2OS cells using BCS1L antibody (STJ29961) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using BCS1L antibody (STJ29961) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using BCS1L antibody (STJ29961) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using BCS1L antibody (STJ29961) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-BCS1L antibody (33-240) (STJ29961)

SKU:
STJ29961

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BCS1L
Gene ID: 617
Uniprot ID: BCS1_HUMAN
Immunogen Region: 33-240
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 33-240 of human BCS1L (NP_004319.1).
Immunogen Sequence: RKGVQLGLVAFRRHYMITLE VPARDRSYAWLLSWLTRHST RTQHLSVETSYLQHESGRIS TKFEFVPSPGNHFIWYRGKW IRVERSREMQMIDLQTGTPW ESVTFTALGTDRKVFFNILE EARELALQQEEGKTVMYTAV GSEWRPFGYPRRRRPLNSVV LQQGLADRIVRDVQEFIDNP KWYTDRGIPYRRGYLLYGPP GCGKSSFI
Tissue Specificity Ubiquitous.
Function Chaperone necessary for the incorporation of Rieske iron-sulfur protein UQCRFS1 into the mitochondrial respiratory chain complex III. Plays an important role in the maintenance of mitochondrial tubular networks, respiratory chain assembly and formation of the LETM1 complex.
Protein Name Mitochondrial Chaperone Bcs1
H-Bcs1
Bcs1-Like Protein
Database Links Reactome: R-HSA-1268020
Reactome: R-HSA-9865881
Cellular Localisation Mitochondrion Inner Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-Mitochondrial Chaperone Bcs1 antibody
Anti-H-Bcs1 antibody
Anti-Bcs1-Like Protein antibody
Anti-BCS1L antibody
Anti-BCS1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance