• Western blot analysis of extracts of MCF7 cells, using Bcdin3d antibody (STJ11103423) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunofluorescence analysis of NIH/3T3 cells using Bcdin3d rabbit polyclonal antibody (STJ11103423) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.

Anti-Bcdin3d antibody (65-197) (STJ11103423)

SKU:
STJ11103423

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Bcdin3d (65-197) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Bcdin3d
Gene ID: 75284
Uniprot ID: BN3D2_MOUSE
Immunogen Region: 65-197
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 65-197 of mouse Bcdin3d (NP_083512.2).
Immunogen Sequence: EKRPILGLDVGCNSGDLSVA LYKHFLSPRDGETCSGASRE LRILCCDIDPVLVERAERDC PFPEALTFITLDIMDQESRK VPLSSFLSQFGRSVFDMVFC MSVTMWIHLNHGDRGLCEFL AHVSSLCSYLLVE
Function O-methyltransferase that specifically monomethylates 5'-monophosphate of cytoplasmic histidyl tRNA (tRNA(His)), acting as a capping enzyme by protecting tRNA(His) from cleavage by DICER1. Also able, with less efficiently, to methylate the 5' monophosphate of a subset of pre-miRNAs, acting as a negative regulator of miRNA processing. The 5' monophosphate of pre-miRNAs is recognized by DICER1 and is required for pre-miRNAs processing: methylation at this position reduces the processing of pre-miRNAs by DICER1. Was also reported to mediate dimethylation of pre-miR-145.however dimethylation cannot be reproduced by another group which observes a monomethylation of pre-miR-145.
Protein Name Rna 5'-Monophosphate Methyltransferase
Bcdin3 Domain-Containing Protein
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Rna 5'-Monophosphate Methyltransferase antibody
Anti-Bcdin3 Domain-Containing Protein antibody
Anti-Bcdin3d antibody

Information sourced from Uniprot.org

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