• Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L. ) variety Zhonghua 11, using BC12 Rabbit polyclonal antibody (STJ11104025) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.

Anti-BC12 antibody (840-970 aa) (STJ11104025)

SKU:
STJ11104025

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Host: Rabbit
Applications: WB/ELISA
Reactivity: O.sativa
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 840-970 aa
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 840-970 of Oryza sativa BC12. (Q6YUL8).
Immunogen Sequence: EAKSLLQYIFNVAADARCQV REKEMEIKEMKEQMTELVTI LRHSESRRRETEKQLKQREQ AAVTATTSPGNGNGSVKHSA DDSNTPLSPVAVPAQKQLKY SAGIVNSPSKGVPAFNKQHL KMVPMAQLPVG
Background Microtubule-dependent motor protein involved in the control of the oriented deposition of cellulose microfibrils.Involved in wall biogenesis and modification, and contributes to cell-cycle progression and cell division. Acts as a transcriptional activator in gibberellic acid (GA) biosynthesis pathway. Binds specifically to the DNA sequence 5 NA-ACCAACTTGAA-3 of the ent-kaurene oxidase 2 (CYP701A6 or OsKO2) promoter. May regulate CYP701A6 gene expression and mediates cell elongation by regulating the GA biosynthesis pathway

Information sourced from Uniprot.org

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