• Western blot analysis of various lysates using B3GNT5 Rabbit polyclonal antibody (STJ116638) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-B3GNT5 antibody (36-378) (STJ116638)

SKU:
STJ116638

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: B3GNT5
Gene ID: 84002
Uniprot ID: B3GN5_HUMAN
Immunogen Region: 36-378
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 36-378 of human B3GNT5 (NP_114436.1).
Immunogen Sequence: DNHIVSHMKSYSYRYLINSY DFVNDTLSLKHTSAGPRYQY LINHKEKCQAQDVLLLLFVK TAPENYDRRSGIRRTWGNEN YVRSQLNANIKTLFALGTPN PLEGEELQRKLAWEDQRYND IIQQDFVDSFYNLTLKLLMQ FSWANTYCPHAKFLMTADDD IFIHMPNLIEYLQSLEQIGV QDFWIGRVHRGAPPIRDKSS KYYVSYEMYQWPAYPDYTAG AAYVISGDVAAKVYEASQT
Tissue Specificity Widely expressed. Highly expressed in lung, colon, placenta, testis, pituitary gland and cerebellum. Weakly expressed in brain, liver, spleen, lymph node and thymus.
Function Beta-1,3-N-acetylglucosaminyltransferase that plays a key role in the synthesis of lacto- or neolacto-series carbohydrate chains on glycolipids, notably by participating in biosynthesis of HNK-1 and Lewis X carbohydrate structures. Has strong activity toward lactosylceramide (LacCer) and neolactotetraosylceramide (nLc(4)Cer.paragloboside), resulting in the synthesis of Lc(3)Cer and neolactopentaosylceramide (nLc(5)Cer), respectively. Probably plays a central role in regulating neolacto-series glycolipid synthesis during embryonic development.
Protein Name Lactosylceramide 1 -3-N-Acetyl-Beta-D-Glucosaminyltransferase
Lactotriaosylceramide Synthase
Lc(3cer Synthase
Lc3 Synthase
Udp-Glcnac -Beta-Gal Beta-1 -3-N-Acetylglucosaminyltransferase 5
Bgnt-5
Beta-1 -3-Gn-T5
Beta-1 -3-N-Acetylglucosaminyltransferase 5
Beta3gn-T5
Database Links Reactome: R-HSA-913709
Reactome: R-HSA-9840309
Cellular Localisation Golgi Apparatus Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-Lactosylceramide 1 -3-N-Acetyl-Beta-D-Glucosaminyltransferase antibody
Anti-Lactotriaosylceramide Synthase antibody
Anti-Lc(3cer Synthase antibody
Anti-Lc3 Synthase antibody
Anti-Udp-Glcnac -Beta-Gal Beta-1 -3-N-Acetylglucosaminyltransferase 5 antibody
Anti-Bgnt-5 antibody
Anti-Beta-1 -3-Gn-T5 antibody
Anti-Beta-1 -3-N-Acetylglucosaminyltransferase 5 antibody
Anti-Beta3gn-T5 antibody
Anti-B3GNT5 antibody

Information sourced from Uniprot.org

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