• Western blot analysis of various lysates, using ATP6 antibody (STJ11105030) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ATP6 antibody [S5030RM] (STJ11105030)

SKU:
STJ11105030

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ATP6 is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S5030RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Mtatp6
Gene ID: 17705
Uniprot ID: ATP6_MOUSE
Immunogen: A synthesized peptide derived from human ATP6.
Function Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Key component of the proton channel.it may play a direct role in the translocation of protons across the membrane.
Protein Name Atp Synthase Subunit A
F-Atpase Protein 6
Database Links Reactome: R-MMU-163210
Reactome: -MMU-8949613
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Atp Synthase Subunit A antibody
Anti-F-Atpase Protein 6 antibody
Anti-Mtatp6 antibody
Anti-Atp6 antibody
Anti-Atpase6 antibody
Anti-mt-Atp6 antibody

Information sourced from Uniprot.org

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