• Immunofluorescence analysis of paraffin-embedded mouse testis using ASZ1 Rabbit polyclonal antibody (STJ116657) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded rat testis using ASZ1 Rabbit polyclonal antibody (STJ116657) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of ASZ1 in paraffin-embedded mouse testis using ASZ1 Rabbit polyclonal antibody (STJ116657) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ASZ1 in paraffin-embedded rat testis using ASZ1 Rabbit polyclonal antibody (STJ116657) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using ASZ1 Rabbit polyclonal antibody (STJ116657) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ASZ1 antibody (1-350) (STJ116657)

SKU:
STJ116657

$151.88 - $705.38
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ASZ1 (1-350) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ASZ1
Gene ID: 136991
Uniprot ID: ASZ1_HUMAN
Immunogen Region: 1-350
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human ASZ1 (NP_570124.1).
Immunogen Sequence: MAASALRGLPVAGGGESSES EDDGWEIGYLDRTSQKLKRL LPIEEKKEKFKKAMTIGDVS LVQELLDSGISVDSNFQYGW TPLMYAASVANAELVRVLLD RGANASFEKDKQSILITACS AHGSEEQILKCVELLLSRNA DPNVACRRLMTPIMYAARDG HTQVVALLVAHGAEVNTQDE NGYTALTWAARQGHKNIVLK LLELGANKMLQTKDGKMPSE IAKRNKHHEIFNLLSFTLN
Tissue Specificity Expressed exclusively in the testis and ovary and at higher levels in the adult testis compared with the adult ovary.
Function Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Its association with pi-bodies suggests a participation in the primary piRNAs metabolic process. Required prior to the pachytene stage to facilitate the production of multiple types of piRNAs, including those associated with repeats involved in the regulation of retrotransposons. May act by mediating protein-protein interactions during germ cell maturation.
Protein Name Ankyrin Repeat - Sam And Basic Leucine Zipper Domain-Containing Protein 1
Ankyrin-Like Protein 1
Germ Cell-Specific Ankyrin - Sam And Basic Leucine Zipper Domain-Containing Protein
Database Links Reactome: R-HSA-5601884
Cellular Localisation Cytoplasm
Component Of The Meiotic Nuage
Also Named P Granule
A Germ-Cell-Specific Organelle Required To Repress Transposon Activity During Meiosis
Specifically Localizes To Pi-Bodies
A Subset Of The Nuage Which Contains Primary Pirnas
Alternative Antibody Names Anti-Ankyrin Repeat - Sam And Basic Leucine Zipper Domain-Containing Protein 1 antibody
Anti-Ankyrin-Like Protein 1 antibody
Anti-Germ Cell-Specific Ankyrin - Sam And Basic Leucine Zipper Domain-Containing Protein antibody
Anti-ASZ1 antibody
Anti-ALP1 antibody
Anti-ANKL1 antibody
Anti-C7orf7 antibody
Anti-GASZ antibody

Information sourced from Uniprot.org

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