• Western blot analysis of extracts of various cell lines, using ASIC5 antibody (STJ112345) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ASIC5 antibody (140-330) (STJ112345)

SKU:
STJ112345

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ASIC5 (140-330) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ASIC5
Gene ID: 51802
Uniprot ID: ASIC5_HUMAN
Immunogen Region: 140-330
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 140-330 of human ASIC5 (NP_059115.1).
Immunogen Sequence: HLQEITANSTGSREATDFAA SHQNFSIVEFIRNKGFYLNN STLLDCEFFGKPCSPKDFAH VFTEYGNCFTFNHGETLQAK RKVSVSGRGLSLLFNVNQEA FTDNPALGFVDAGIIFVIHS PKKVPQFDGLGLLSPVGMHA RVTIRQVKTVHQEYPWGECN PNIKLQNFSSYSTSGCLKEC KAQHIKKQCGC
Tissue Specificity Detected in small intestine, duodenum and jejunum. Detected at very low levels in testis and rectum.
Function Cation channel that gives rise to very low constitutive currents in the absence of activation. The activated channel exhibits selectivity for sodium, and is inhibited by amiloride.
Protein Name Acid-Sensing Ion Channel 5
Asic5
Amiloride-Sensitive Cation Channel 5
Human Intestine Na(+ Channel
Hinac
Database Links Reactome: R-HSA-2672351
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Acid-Sensing Ion Channel 5 antibody
Anti-Asic5 antibody
Anti-Amiloride-Sensitive Cation Channel 5 antibody
Anti-Human Intestine Na(+ Channel antibody
Anti-Hinac antibody
Anti-ASIC5 antibody
Anti-ACCN5 antibody
Anti-HINAC antibody

Information sourced from Uniprot.org

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