| Host: |
NZ White Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Nz White Rabbit polyclonal antibody anti-ASIC3 (50-100) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Shipped as lyophilised. Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material. |
| Purification: |
Ammonium sulphate precipitation |
| Dilution Range: |
A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
Asic3 |
| Gene ID: |
171209 |
| Uniprot ID: |
ASIC3_MOUSE |
| Immunogen Region: |
50-100 |
| Specificity: |
Specific for ASIC3. |
| Immunogen: |
A synthetic peptide from aa region 50-100 of rat ASIC3 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse. |
| Post Translational Modifications | Phosphorylated by PKA. Phosphorylated by PKC. In vitro, PRKCABP/PICK-1 is necessary for PKC phosphorylation and activation of a ASIC3/ACCN3-ASIC2/ASIC2b channel, but does not activate a homomeric ASIC3/ACCN3 channel. |
| Function | Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Generates a biphasic current with a fast inactivating and a slow sustained phase. In sensory neurons is proposed to mediate the pain induced by acidosis that occurs in ischemic, damaged or inflamed tissue. May be involved in hyperalgesia. May play a role in mechanoreception. Heteromeric channel assembly seems to modulate channel properties. |
| Protein Name | Acid-Sensing Ion Channel 3Asic3Amiloride-Sensitive Cation Channel 3Dorsal Root AsicDrasic |
| Database Links | Reactome: R-MMU-2672351 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinCytoplasmCell Surface Expression May Be Stabilized By Interaction With Lin7b And Cytoplasmic Retention By Interaction With Dlg4In Part Cytoplasmic In Cochlea Cells |
| Alternative Antibody Names | Anti-Acid-Sensing Ion Channel 3 antibodyAnti-Asic3 antibodyAnti-Amiloride-Sensitive Cation Channel 3 antibodyAnti-Dorsal Root Asic antibodyAnti-Drasic antibodyAnti-Asic3 antibodyAnti-Accn3 antibodyAnti-Drasic antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
