• Western blot analysis of extracts of U-251MG cells, using ARPC1A antibody (STJ116713) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ARPC1A antibody (171-350) (STJ116713)

SKU:
STJ116713

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ARPC1A (171-350) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARPC1A
Gene ID: 10552
Uniprot ID: ARC1A_HUMAN
Immunogen Region: 171-350
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 171-350 of human ARPC1A (NP_006400.2).
Immunogen Sequence: AYIKEVDEKPASTPWGSKMP FGQLMSEFGGSGTGGWVHGV SFSASGSRLAWVSHDSTVSV ADASKSVQVSTLKTEFLPLL SVSFVSENSVVAAGHDCCPM LFNYDDRGCLTFVSKLDIPK QSIQRNMSAMERFRNMDKRA TTEDRNTALETLHQNSITQV SIYEVDKQDCRKFCTTGIDG
Function Probably functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.
Protein Name Actin-Related Protein 2/3 Complex Subunit 1a
Sop2-Like Protein
Database Links Reactome: R-HSA-2029482
Reactome: R-HSA-3928662
Reactome: R-HSA-5663213
Reactome: R-HSA-8856828
Reactome: R-HSA-9664422
Cellular Localisation Cytoplasm
Cytoskeleton
Nucleus
Alternative Antibody Names Anti-Actin-Related Protein 2/3 Complex Subunit 1a antibody
Anti-Sop2-Like Protein antibody
Anti-ARPC1A antibody
Anti-SOP2L antibody

Information sourced from Uniprot.org

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