| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S4MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:1000-1:2000 IHC-P:1:800-1:8000 IF/ICC:1:100-1:400 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CYP19A1 |
| Gene ID: | 1588 |
| Uniprot ID: | CP19A_HUMAN |
| Immunogen Region: | 1-100 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Aromatase (CYP19A1) (P11511). |
| Immunogen Sequence: | MVLEMLNPIHYNITSIVPEA MPAATMPVLLLTGLFLLVWN YEGTSSIPGPGYCMGIGPLI SHGRFLWMGIGSACNYYNRV YGEFMRVWISGEETLIISKS |
| Tissue Specificity | Widely expressed, including in adult and fetal brain, placenta, skin fibroblasts, adipose tissue and gonads. |
| Post Translational Modifications | Phosphorylated in vitro by PKA and PKG/PRKG1. These phosphorylations inhibit the catalytic activity as measured by estrone synthesis from androstenedione (36% decrease for PKA and 30% for PKG/PRKG1). |
| Function | A cytochrome P450 monooxygenase that catalyzes the conversion of C19 androgens, androst-4-ene-3,17-dione (androstenedione) and testosterone to the C18 estrogens, estrone and estradiol, respectively. Catalyzes three successive oxidations of C19 androgens: two conventional oxidations at C19 yielding 19-hydroxy and 19-oxo/19-aldehyde derivatives, followed by a third oxidative aromatization step that involves C1-beta hydrogen abstraction combined with cleavage of the C10-C19 bond to yield a phenolic A ring and formic acid. Alternatively, the third oxidative reaction yields a 19-norsteroid and formic acid. Converts dihydrotestosterone to delta1,10-dehydro 19-nordihydrotestosterone and may play a role in homeostasis of this potent androgen. Also displays 2-hydroxylase activity toward estrone. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR.NADPH-ferrihemoprotein reductase). |
| Protein Name | Aromatase Cypxix Cytochrome P-450arom Cytochrome P450 19a1 Estrogen Synthase |
| Database Links | Reactome: R-HSA-193144 Reactome: R-HSA-211976 Reactome: R-HSA-5579030 |
| Cellular Localisation | Endoplasmic Reticulum Membrane Multi-Pass Membrane Protein Microsome Membrane |
| Alternative Antibody Names | Anti-Aromatase antibody Anti-Cypxix antibody Anti-Cytochrome P-450arom antibody Anti-Cytochrome P450 19a1 antibody Anti-Estrogen Synthase antibody Anti-CYP19A1 antibody Anti-ARO1 antibody Anti-CYAR antibody Anti-CYP19 antibody |
Information sourced from Uniprot.org