| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ARFGEF2 (220-370) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:200-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ARFGEF2 |
| Gene ID: |
10564 |
| Uniprot ID: |
BIG2_HUMAN |
| Immunogen Region: |
220-370 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 220-370 of human ARFGEF2 (NP_006411.2). |
| Immunogen Sequence: |
VIQAAAVSPKFVRLKHSQAQ SKPTTPEKTDLTNGEHARSD SGKVSTENGDAPRERGSSLS GTDDGAQEVVKDILEDVVTS AIKEAAEKHGLTEPERVLGE LECQECAIPPGVDENSQTNG IADDRQSLSSADNLESDAQG HQVAARFSHVL |
| Tissue Specificity | Expressed in placenta, lung, heart, brain, kidney and pancreas. |
| Post Translational Modifications | In vitro phosphorylated by PKA reducing its GEF activity and dephosphorylated by phosphatase PP1. |
| Function | Promotes guanine-nucleotide exchange on ARF1 and ARF3 and to a lower extent on ARF5 and ARF6. Promotes the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP. Involved in the regulation of Golgi vesicular transport. Required for the integrity of the endosomal compartment. Involved in trafficking from the trans-Golgi network (TGN) to endosomes and is required for membrane association of the AP-1 complex and GGA1. Seems to be involved in recycling of the transferrin receptor from recycling endosomes to the plasma membrane. Probably is involved in the exit of GABA(A) receptors from the endoplasmic reticulum. Involved in constitutive release of tumor necrosis factor receptor 1 via exosome-like vesicles.the function seems to involve PKA and specifically PRKAR2B. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways. |
| Protein Name | Brefeldin A-Inhibited Guanine Nucleotide-Exchange Protein 2Brefeldin A-Inhibited Gep 2Adp-Ribosylation Factor Guanine Nucleotide-Exchange Factor 2 |
| Database Links | Reactome: R-HSA-390471 |
| Cellular Localisation | CytoplasmMembraneGolgi ApparatusPerinuclear RegionTrans-Golgi NetworkEndosomeCytoskeletonMicrotubule Organizing CenterCentrosomeCell ProjectionDendriteCytoplasmic VesicleSynapseTranslocates From Cytoplasm To Membranes Upon Camp TreatmentLocalized In Recycling Endosomes |
| Alternative Antibody Names | Anti-Brefeldin A-Inhibited Guanine Nucleotide-Exchange Protein 2 antibodyAnti-Brefeldin A-Inhibited Gep 2 antibodyAnti-Adp-Ribosylation Factor Guanine Nucleotide-Exchange Factor 2 antibodyAnti-ARFGEF2 antibodyAnti-ARFGEP2 antibodyAnti-BIG2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
