• Western blot analysis of extracts of various cell lines, using Aquaporin-4 (AQP4) antibody (STJ115861) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-AQP4 antibody (244-323) (STJ115861)

SKU:
STJ115861

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Aquaporin 4 (244-323) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AQP4
Gene ID: 361
Uniprot ID: AQP4_HUMAN
Immunogen Region: 244-323
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 244-323 of human Aquaporin-4 (AQP4) (NP_001641.1).
Immunogen Sequence: AGGLYEYVFCPDVEFKRRFK EAFSKAAQQTKGSYMEVEDN RSQVETDDLILKPGVVHVID VDRGEEKKGKDQSGEVLSSV
Tissue Specificity Detected in skeletal muscle. Detected in stomach, along the glandular base region of the fundic gland (at protein level). Detected in brain, lung and skeletal muscle, and at much lower levels in heart and ovary.
Post Translational Modifications Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamate increases conductance by 40%. Isoform 2: Palmitoylated on its N-terminal region. Isoform 1: Not palmitoylated.
Function Forms a water-specific channel. Plays an important role in brain water homeostasis and in glymphatic solute transport. Required for a normal rate of water exchange across the blood brain interface. Required for normal levels of cerebrospinal fluid influx into the brain cortex and parenchyma along paravascular spaces that surround penetrating arteries, and for normal drainage of interstitial fluid along paravenous drainage pathways. Thereby, it is required for normal clearance of solutes from the brain interstitial fluid, including soluble beta-amyloid peptides derived from APP. Plays a redundant role in urinary water homeostasis and urinary concentrating ability.
Protein Name Aquaporin-4
Aqp-4
Mercurial-Insensitive Water Channel
Miwc
Wch4
Database Links Reactome: R-HSA-432040
Reactome: R-HSA-432047
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Basolateral Cell Membrane
Endosome Membrane
Sarcolemma
Cell Projection
Activation Of The Vasopressin Receptor Avpr1a Triggers Aqp4 Phosphorylation At Ser-180 And Promotes Its Internalization From The Cell Membrane
Detected On Brain Astrocyte Processes And Astrocyte Endfeet Close To Capillaries
Alternative Antibody Names Anti-Aquaporin-4 antibody
Anti-Aqp-4 antibody
Anti-Mercurial-Insensitive Water Channel antibody
Anti-Miwc antibody
Anti-Wch4 antibody
Anti-AQP4 antibody

Information sourced from Uniprot.org

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