• Western blot analysis of extracts of various cell lines, using APOD antibody (STJ118087) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-APOD antibody (21-189) (STJ118087)

SKU:
STJ118087

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-APOD (21-189) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: APOD
Gene ID: 347
Uniprot ID: APOD_HUMAN
Immunogen Region: 21-189
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 21-189 of human APOD (NP_001638.1).
Immunogen Sequence: QAFHLGKCPNPPVQENFDVN KYLGRWYEIEKIPTTFENGR CIQANYSLMENGKIKVLNQE LRADGTVNQIEGEATPVNLT EPAKLEVKFSWFMPSAPYWI LATDYENYALVYSCTCIIQL FHVDFAWILARNPNLPPETV DSLKNILTSNNIDVKKMTVT DQVNCPKLS
Tissue Specificity Expressed in liver, intestine, pancreas, kidney, placenta, adrenal, spleen, fetal brain tissue and tears.
Post Translational Modifications N-glycosylatd. N-glycan heterogeneity at Asn-65: Hex5HexNAc4 (major) and Hex6HexNAc5 (minor).at Asn-98: Hex5HexNAc4 (minor), dHex1Hex5HexNAc4 (major), dHex1Hex6HexNAc5 (minor) and dHex1Hex7HexNAc6 (minor).
Function APOD occurs in the macromolecular complex with lecithin-cholesterol acyltransferase. It is probably involved in the transport and binding of bilin. Appears to be able to transport a variety of ligands in a number of different contexts.
Protein Name Apolipoprotein D
Apo-D
Apod
Database Links Reactome: R-HSA-804914
Reactome: R-HSA-9029569
Cellular Localisation Secreted
Alternative Antibody Names Anti-Apolipoprotein D antibody
Anti-Apo-D antibody
Anti-Apod antibody
Anti-APOD antibody

Information sourced from Uniprot.org

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