• Immunofluorescence analysis of NIH/3T3 cells using AP1G1 Rabbit polyclonal antibody (STJ11103312) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using AP1G1 Rabbit polyclonal antibody (STJ11103312) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-AP1G1 antibody (1-240) (STJ11103312)

SKU:
STJ11103312

£169.50 - £449.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AP1G1 (1-240) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AP1G1
Gene ID: 164
Uniprot ID: AP1G1_HUMAN
Immunogen Region: 1-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human AP1G1 (NP_001025178).
Immunogen Sequence: MPAPIRLRELIRTIRTARTQ AEEREMIQKECAAIRSSFRE EDNTYRCRNVAKLLYMHMLG YPAHFGQLECLKLIASQKFT DKRIGYLGAMLLLDERQDVH LLMTNCIKNDLNHSTQFVQG LALCTLGCMGSSEMCRDLAG EVEKLLKTSNSYLRKKAALC AVHVIRKVPELMEMFLPATK NLLNEKNHGVLHTSVVLLTE MCERSPDMLAHFRKNEKLVP QLVRILKNLIMSGYSPEHD
Tissue Specificity Widely expressed.
Function Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. In association with AFTPH/aftiphilin in the aftiphilin/p200/gamma-synergin complex, involved in the trafficking of transferrin from early to recycling endosomes, and the membrane trafficking of furin and the lysosomal enzyme cathepsin D between the trans-Golgi network (TGN) and endosomes.
Protein Name Ap-1 Complex Subunit Gamma-1
Adaptor Protein Complex Ap-1 Subunit Gamma-1
Adaptor-Related Protein Complex 1 Subunit Gamma-1
Clathrin Assembly Protein Complex 1 Gamma-1 Large Chain
Gamma1-Adaptin
Golgi Adaptor Ha1/Ap1 Adaptin Subunit Gamma-1
Database Links Reactome: R-HSA-164940
Reactome: R-HSA-2132295
Reactome: R-HSA-432720
Reactome: R-HSA-432722
Cellular Localisation Golgi Apparatus
Cytoplasmic Vesicle
Clathrin-Coated Vesicle Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytoplasm
Perinuclear Region
Clathrin-Coated Vesicle
Membrane
Clathrin-Coated Pit
Component Of The Coat Surrounding The Cytoplasmic Face Of Coated Vesicles Located At The Golgi Complex
Co-Localizes With Aftph/Aftiphilin In The Cytoplasm
Alternative Antibody Names Anti-Ap-1 Complex Subunit Gamma-1 antibody
Anti-Adaptor Protein Complex Ap-1 Subunit Gamma-1 antibody
Anti-Adaptor-Related Protein Complex 1 Subunit Gamma-1 antibody
Anti-Clathrin Assembly Protein Complex 1 Gamma-1 Large Chain antibody
Anti-Gamma1-Adaptin antibody
Anti-Golgi Adaptor Ha1/Ap1 Adaptin Subunit Gamma-1 antibody
Anti-AP1G1 antibody
Anti-ADTG antibody
Anti-CLAPG1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance