• Western blot analysis of various lysates using ANO5 Rabbit polyclonal antibody (STJ117412) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ANO5 antibody (1-300) (STJ117412)

SKU:
STJ117412

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ANO5 (1-300) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ANO5
Gene ID: 203859
Uniprot ID: ANO5_HUMAN
Immunogen Region: 1-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ANO5 (NP_998764.1).
Immunogen Sequence: MGDPDLLEVLAEEGEKVNKH IDYSFQMSEQSLSSRETSFL INEETMPAKRFNLFLRRRLM FQKNQQSKDSIFFRDGIRQI DFVLSYVDDVKKDAELKAER RKEFETNLRKTGLELEIEDK RDSEDGRTYFVKIHAPWEVL VTYAEVLGIKMPIKESDIPR PKHTPISYVLGPVRLPLSVK YPHPEYFTAQFSRHRQELFL IEDQATFFPSSSRNRIVYYI LSRCPFGIEDGKKRFGIER
Tissue Specificity Highly expressed in brain, heart, kidney, lung, and skeletal muscle. Weakly expressed in bone marrow, fetal liver, placenta, spleen, thymus, osteoblasts and periodontal ligament cells.
Function Does not exhibit calcium-activated chloride channel (CaCC) activity.
Protein Name Anoctamin-5
Gnathodiaphyseal Dysplasia 1 Protein
Transmembrane Protein 16e
Database Links Reactome: R-HSA-2672351
Reactome: R-HSA-9733458
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Cell Membrane
Colocalized With Calr/Calreticulin
Shows An Intracellular Localization
Alternative Antibody Names Anti-Anoctamin-5 antibody
Anti-Gnathodiaphyseal Dysplasia 1 Protein antibody
Anti-Transmembrane Protein 16e antibody
Anti-ANO5 antibody
Anti-GDD1 antibody
Anti-TMEM16E antibody

Information sourced from Uniprot.org

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