• Western blot analysis of extracts of various cell lines, using ANKRD13A antibody (STJ113670) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ANKRD13A antibody (1-240) (STJ113670)

SKU:
STJ113670

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ANKRD13A (1-240) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ANKRD13A
Gene ID: 88455
Uniprot ID: AN13A_HUMAN
Immunogen Region: 1-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human ANKRD13A (NP_149112.1).
Immunogen Sequence: MSSACDAGDHYPLHLLVWKN DYRQLEKELQGQNVEAVDPR GRTLLHLAVSLGHLESARVL LRHKADVTKENRQGWTVLHE AVSTGDPEMVYTVLQHRDYH NTSMALEGVPELLQKILEAP DFYVQMKWEFTSWVPLVSRI CPNDVCRIWKSGAKLRVDIT LLGFENMSWIRGRRSFIFKG EDNWAELMEVNHDDKVVTTE RFDLSQEMERLTLDLMKPKS REVERRLTSPVINTSLDTK
Post Translational Modifications Monoubiquitinated, inhibits interaction with ubiquitinated EGFR.
Function Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Does not bind 'Lys-48'-linked ubiquitin. Positively regulates the internalization of ligand-activated EGFR by binding to the Ub moiety of ubiquitinated EGFR at the cell membrane.
Protein Name Ankyrin Repeat Domain-Containing Protein 13a
Protein Ke03
Cellular Localisation Cell Membrane
Late Endosome
Interaction With Egfr May Enhance Association With The Cell Membrane
Alternative Antibody Names Anti-Ankyrin Repeat Domain-Containing Protein 13a antibody
Anti-Protein Ke03 antibody
Anti-ANKRD13A antibody
Anti-ANKRD13 antibody

Information sourced from Uniprot.org

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