• Immunofluorescence analysis of U-251 MG cells using AMPH Rabbit polyclonal antibody (STJ27342) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using AMPH Polyclonal Antibody (STJ27342) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-AMPH antibody (1-250) (STJ27342)

SKU:
STJ27342

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AMPH (1-250) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AMPH
Gene ID: 273
Uniprot ID: AMPH_HUMAN
Immunogen Region: 1-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human AMPH (NP_001626.1).
Immunogen Sequence: MADIKTGIFAKNVQKRLNRA QEKVLQKLGKADETKDEQFE EYVQNFKRQEAEGTRLQREL RGYLAAIKGMQEASMKLTES LHEVYEPDWYGREDVKMVGE KCDVLWEDFHQKLVDGSLLT LDTYLGQFPDIKNRIAKRSR KLVDYDSARHHLEALQSSKR KDESRISKAEEEFQKAQKVF EEFNVDLQEELPSLWSRRVG FYVNTFKNVSSLEAKFHKEI AVLCHKLYEVMTKLGDQHA
Tissue Specificity Neurons, certain endocrine cell types and spermatocytes.
Function May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.
Protein Name Amphiphysin
Database Links Reactome: R-HSA-8856828
Cellular Localisation Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Amphiphysin antibody
Anti-AMPH antibody
Anti-AMPH1 antibody

Information sourced from Uniprot.org

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