• Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma using a-tubulin (Acetyl Lys40) Mouse mAb diluted at 1:200.
  • Immunohistochemical analysis of paraffin-embedded Mouse Brain Tissue using a-tubulin (Acetyl Lys40) Mouse mAb diluted at 1:200.
  • Immunofluorescence analysis of Rat-spinal-cord tissue. 1, Alpha-tubulin (Acetyl Lys40) monoclonal antibody (4A8) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Western blot analysis of extracts from Hela cells, untreated (-) or treated with TSA (1 Mu M, 18 hr; +), using Acetyl-a-tubulin (Lys40) Mouse mAb 1:2000.
  • Immunofluorescence analysis of Mouse-lung tissue. 1, Alpha-tubulin (Acetyl Lys40) monoclonal antibody (4A8) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of Human-liver-cancer tissue. 1, Alpha-tubulin (Acetyl Lys40) monoclonal antibody (4A8) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1, Alpha-tubulin (Acetyl Lys40) monoclonal antibody (4A8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1, Alpha-tubulin (Acetyl Lys40) monoclonal antibody (4A8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, Alpha-tubulin (Acetyl Lys40) monoclonal antibody (4A8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-alpha-tubulin (Acetyl Lys40) antibody (STJ97537)

SKU:
STJ97537
Current Stock:
Host: Mouse
Applications: WB, IF, IHC-P
Reactivity: Human, Rat, Mouse
Short Description: Mouse monoclonal primary antibody against alpha-tubulin (Acetyl Lys40).
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Monoclonal
Clone ID: 4A8
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using a specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG1
Dilution Range: WB 1:1000-2000
IHC 1:50-100
IF 1:200
Specificity: alpha-tubulin (Acetyl Lys40) protein detects endogenous levels of alpha-tubulin (Acetyl Lys40)
Uniprot ID: TBA4A_HUMAN
Gene ID: 7277
Gene Symbol: TUBA4A
Immunogen: Synthetic Peptide
Post Translational Modifications Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold. Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable). Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly. Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability.
Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Database Links Reactome: R-HSA-114608
Cellular Localisation Cytoplasm
Cytoskeleton
Protein Name Tubulin Alpha-4a Chain
Alpha-Tubulin 1
Testis-Specific Alpha-Tubulin
Tubulin H2-Alpha
Tubulin Alpha-1 Chain
Alternative Names Anti-Tubulin Alpha-4a Chain antibody
Anti-Alpha-Tubulin 1 antibody
Anti-Testis-Specific Alpha-Tubulin antibody
Anti-Tubulin H2-Alpha antibody
Anti-Tubulin Alpha-1 Chain antibody
Anti-TUBA4A antibody
Anti-TUBA1 antibody

Information sourced from Uniprot.org

6 months standard