Western blot analysis of extracts of various cell lines, using ALKBH4 Antibody (STJ110122) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ALKBH4 antibody (1-170) (STJ110122)

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STJ110122
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ALKBH4
Gene ID: 54784
Uniprot ID: ALKB4_HUMAN
Immunogen Region: 1-170
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human ALKBH4 (NP_060091.1).
Immunogen Sequence: MAAAAAETPEVLRECGCKGI RTCLICERQRGSDPPWELPP AKTYRFIYCSDTGWAVGTEE SDFEGWAFPFPGVMLIEDFV TREEEAELVRLMDRDPWKLS QSGRRKQDYGPKVNFRKQKL KTEGFCGLPSFSREVVRRMG LYPGLEGFRPVEQCNLDYCP ERGSAIDPHL
Tissue Specificity Widely expressed, with highest expression in pancreas, ovary and spleen.
Function Dioxygenase that mediates demethylation of actin monomethylated at 'Lys-84' (K84me1), thereby acting as a regulator of actomyosin-processes. Demethylation of actin K84me1 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. In addition to proteins, also demethylates DNA: specifically demethylates DNA methylated on the 6th position of adenine (N(6)-methyladenosine) DNA, thereby regulating Polycomb silencing.
Protein Name Alpha-Ketoglutarate-Dependent Dioxygenase Alkb Homolog 4
Alkylated Dna Repair Protein Alkb Homolog 4
Dna N6-Methyl Adenine Demethylase Alkbh4
Lysine-Specific Demethylase Alkbh4
Cellular Localisation Cytoplasm
Nucleus
Nucleolus
Midbody
Associates With The Contractile Ring And Midbody
Alternative Antibody Names Anti-Alpha-Ketoglutarate-Dependent Dioxygenase Alkb Homolog 4 antibody
Anti-Alkylated Dna Repair Protein Alkb Homolog 4 antibody
Anti-Dna N6-Methyl Adenine Demethylase Alkbh4 antibody
Anti-Lysine-Specific Demethylase Alkbh4 antibody
Anti-ALKBH4 antibody
Anti-ABH4 antibody

Information sourced from Uniprot.org

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