| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ALKBH2 (1-261) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ALKBH2 |
| Gene ID: |
121642 |
| Uniprot ID: |
ALKB2_HUMAN |
| Immunogen Region: |
1-261 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human ALKBH2 (NP_001138847.1). |
| Immunogen Sequence: |
MDRFLVKGAQGGLLRKQEEQ EPTGEEPAVLGGDKESTRKR PRREAPGNGGHSAGPSWRHI RAEGLDCSYTVLFGKAEADE IFQELEKEVEYFTGALARVQ VFGKWHSVPRKQATYGDAGL TYTFSGLTLSPKPWIPVLER IRDHVSGVTGQTFNFVLINR YKDGCDHIGEHRDDERELAP GSPIASVSFGACRDFVFRHK DSRGKSPSRRVAVVRLPLAH GSLLMMNHPTNTHWYHSLP |
| Tissue Specificity | Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder. |
| Function | Dioxygenase that repairs alkylated nucleic acid bases by direct reversal oxidative dealkylation. Can process both double-stranded (ds) and single-stranded (ss) DNA substrates, with a strong preference for dsDNA. Uses molecular oxygen, 2-oxoglutarate and iron as cofactors to oxidize the alkyl groups that are subsequently released as aldehydes, regenerating the undamaged bases. Probes the base pair stability, locates a weakened base pair and flips the damaged base to accommodate the lesion in its active site for efficient catalysis. Repairs monoalkylated bases, specifically N1-methyladenine and N3-methylcytosine, as well as higher order alkyl adducts such as bases modified with exocyclic bridged adducts known as etheno adducts including 1,N6-ethenoadenine, 3,N4-ethenocytosine and 1,N2-ethenoguanine. Acts as a gatekeeper of genomic integrity under alkylation stress. Efficiently repairs alkylated lesions in ribosomal DNA (rDNA). These lesions can cause ss- and dsDNA strand breaks that severely impair rDNA transcription. In a response mechanism to DNA damage, associates with PCNA at replication forks to repair alkylated adducts prior to replication. |
| Protein Name | Dna Oxidative Demethylase Alkbh2Alkylated Dna Repair Protein Alkb Homolog 2Alpha-Ketoglutarate-Dependent Dioxygenase Alkb Homolog 2Oxy Dc1 |
| Database Links | Reactome: R-HSA-112122 |
| Cellular Localisation | NucleusNucleolusNucleoplasmRelocates To The Replication Foci During S-Phase |
| Alternative Antibody Names | Anti-Dna Oxidative Demethylase Alkbh2 antibodyAnti-Alkylated Dna Repair Protein Alkb Homolog 2 antibodyAnti-Alpha-Ketoglutarate-Dependent Dioxygenase Alkb Homolog 2 antibodyAnti-Oxy Dc1 antibodyAnti-ALKBH2 antibodyAnti-ABH2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
