• Western blot analysis of extracts of various cell lines, using ALG2 antibody (STJ110153) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. _Lysates/proteins: 25ug per lane. _Blocking buffer: 3% non-fat dry milk in TBST. _Detection: ECL Basic Kit. _Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using ALG2 antibody (STJ110153) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ALG2 antibody (197-416) (STJ110153)

SKU:
STJ110153

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ALG2 (197-416) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ALG2
Gene ID: 85365
Uniprot ID: ALG2_HUMAN
Immunogen Region: 197-416
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 197-416 of human ALG2 (NP_149078.1).
Immunogen Sequence: DVLYPSLNVTSFDSVVPEKL DDLVPKGKKFLLLSINRYER KKNLTLALEALVQLRGRLTS QDWERVHLIVAGGYDERVLE NVEHYQELKKMVQQSDLGQY VTFLRSFSDKQKISLLHSCT CVLYTPSNEHFGIVPLEAMY MQCPVIAVNSGGPLESIDHS VTGFLCEPDPVHFSEAIEKF IREPSLKATMGLAGRARVKE KFSPEAFTEQLYRYVTKLLV
Function Mannosylates Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate.
Protein Name Alpha-1 -3/1 -6-Mannosyltransferase Alg2
Asparagine-Linked Glycosylation Protein 2 Homolog
Gdp-Man -Man(1glcnac(2-Pp-Dol Alpha-1 -3-Mannosyltransferase
Gdp-Man -Man(1glcnac(2-Pp-Dolichol Mannosyltransferase
Gdp-Man -Man(2glcnac(2-Pp-Dol Alpha-1 -6-Mannosyltransferase
Database Links Reactome: R-HSA-446193
Reactome: R-HSA-4549349
Cellular Localisation Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-Alpha-1 -3/1 -6-Mannosyltransferase Alg2 antibody
Anti-Asparagine-Linked Glycosylation Protein 2 Homolog antibody
Anti-Gdp-Man -Man(1glcnac(2-Pp-Dol Alpha-1 -3-Mannosyltransferase antibody
Anti-Gdp-Man -Man(1glcnac(2-Pp-Dolichol Mannosyltransferase antibody
Anti-Gdp-Man -Man(2glcnac(2-Pp-Dol Alpha-1 -6-Mannosyltransferase antibody
Anti-ALG2 antibody
Anti-UNQ666 antibody
Anti-PRO1298 antibody

Information sourced from Uniprot.org

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