• Confocal imaging of Mouse liver using ALDH1L1 Rabbit monoclonal antibody (STJ11102338, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using ALDH1L1 Rabbit monoclonal antibody (STJ11102338) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver using ALDH1L1 Rabbit monoclonal antibody (STJ11102338) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using ALDH1L1 Rabbit monoclonal antibody (STJ11102338) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ALDH1L1 antibody (180-300) [S8MR] (STJ11102338)

SKU:
STJ11102338

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ALDH1L1 (180-300) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ALDH1L1
Gene ID: 10840
Uniprot ID: AL1L1_HUMAN
Immunogen Region: 180-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 180-300 of human ALDH1L1 (O75891).
Immunogen Sequence: VRLIAEGKAPRLPQPEEGAT YEGIQKKETAKINWDQPAEA IHNWIRGNDKVPGAWTEACE QKLTFFNSTLNTSGLVPEGD ALPIPGAHRPGVVTKAGLIL FGNDDKMLLVKNIQLEDGKM I
Tissue Specificity Highly expressed in liver, pancreas and kidney.
Post Translational Modifications Phosphopantetheinylation at Ser-354 by AASDHPPT is required for the formyltetrahydrofolate dehydrogenase activity.
Function Cytosolic 10-formyltetrahydrofolate dehydrogenase that catalyzes the NADP(+)-dependent conversion of 10-formyltetrahydrofolate to tetrahydrofolate and carbon dioxide. May also have an NADP(+)-dependent aldehyde dehydrogenase activity towards formaldehyde, acetaldehyde, propionaldehyde, and benzaldehyde.
Protein Name Cytosolic 10-Formyltetrahydrofolate Dehydrogenase
10-Fthfdh
Fdh
Aldehyde Dehydrogenase Family 1 Member L1
Database Links Reactome: R-HSA-196757
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Cytosolic 10-Formyltetrahydrofolate Dehydrogenase antibody
Anti-10-Fthfdh antibody
Anti-Fdh antibody
Anti-Aldehyde Dehydrogenase Family 1 Member L1 antibody
Anti-ALDH1L1 antibody
Anti-FTHFD antibody

Information sourced from Uniprot.org

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