• Western blot analysis of extracts of HepG2 cells, using AJUBA antibody (STJ11100123) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of Rat kidney, using AJUBA antibody (STJ11100123) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-AJUBA antibody (1-100) (STJ11100123)

SKU:
STJ11100123

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AJUBA (1-100) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AJUBA
Gene ID: 84962
Uniprot ID: AJUBA_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Ajuba (NP_116265.1).
Immunogen Sequence: MERLGEKASRLLEKFGRRKG ESSRSGSDGTPGPGKGRLSG LGGPRKSGPRGATGGPGDEP LEPAREQGSLDAERNQRGSF EAPRYEGSFPAGPPPTRALP
Post Translational Modifications Phosphorylated by LATS2 during mitosis. Phosphorylated by AURKA.
Function Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, mitosis, cell-cell adhesion, cell differentiation, proliferation and migration. Contributes to the linking and/or strengthening of epithelia cell-cell junctions in part by linking adhesive receptors to the actin cytoskeleton. May be involved in signal transduction from cell adhesion sites to the nucleus. Plays an important role in regulation of the kinase activity of AURKA for mitotic commitment. Also a component of the IL-1 signaling pathway modulating IL-1-induced NFKB1 activation by influencing the assembly and activity of the PRKCZ-SQSTM1-TRAF6 multiprotein signaling complex. Functions as an HDAC-dependent corepressor for a subset of GFI1 target genes. Acts as a transcriptional corepressor for SNAI1 and SNAI2/SLUG-dependent repression of E-cadherin transcription. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. Positively regulates microRNA (miRNA)-mediated gene silencing. Negatively regulates the Hippo signaling pathway and antagonizes phosphorylation of YAP1.
Protein Name Lim Domain-Containing Protein Ajuba
Database Links Reactome: R-HSA-1234176
Reactome: R-HSA-2565942
Reactome: R-HSA-5617472
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Membrane
Cell Junction
Nucleus
Microtubule Organizing Center
Centrosome
P-Body
Shuttles Between The Cytoplasm And The Nucleus
Localizes On Centrosomes During G2-M Phase
Preferentially Co- Localizes With Cadherin-Adhesive Complexes At Sites Of Cell-Cell Contacts
Colocalizes With Gfi1 In The Nucleus
Alternative Antibody Names Anti-Lim Domain-Containing Protein Ajuba antibody
Anti-AJUBA antibody
Anti-JUB antibody

Information sourced from Uniprot.org

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