• Western blot analysis of extracts of various cell lines, using ADRA1A antibody (STJ110183) at 1:500 dilution. _Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. _Lysates/proteins: 25ug per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 30s.
  • Immunohistochemistry of paraffin-embedded rat lung using ADRA1A antibody (STJ110183) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat heart using ADRA1A antibody (STJ110183) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse heart using ADRA1A antibody (STJ110183) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ADRA1A antibody (100-200) (STJ110183)

SKU:
STJ110183

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ADRA1A (100-200) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADRA1A
Gene ID: 148
Uniprot ID: ADA1A_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human ADRA1A (P35348).
Immunogen Sequence: NIWAAVDVLCCTASIMGLCI ISIDRYIGVSYPLRYPTIVT QRRGLMALLCVWALSLVISI GPLFGWRQPAPEDETICQIN EEPGYVLFSALGSFYLPLAI I
Tissue Specificity Expressed in heart, brain, liver and prostate, but not in kidney, lung, adrenal, aorta and pituitary. Within the prostate, expressed in the apex, base, periurethral and lateral lobe. Isoform 4 is the most abundant isoform expressed in the prostate with high levels also detected in liver and heart.
Post Translational Modifications C-terminal Ser or Thr residues may be phosphorylated.
Function This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine(PE)-stimulated ERK signaling in cardiac myocytes.
Protein Name Alpha-1a Adrenergic Receptor
Alpha-1a Adrenoreceptor
Alpha-1a Adrenoceptor
Alpha-1c Adrenergic Receptor
Alpha-Adrenergic Receptor 1c
Database Links Reactome: R-HSA-390696
Reactome: R-HSA-416476
Reactome: R-HSA-416482
Cellular Localisation Nucleus Membrane
Multi-Pass Membrane Protein
Cell Membrane
Cytoplasm
Membrane
Caveola
Location At The Nuclear Membrane Facilitates Heterooligomerization And Regulates Erk-Mediated Signaling In Cardiac Myocytes
Colocalizes With Gnaq
Plcb1 As Well As Lap2 At The Nuclear Membrane Of Cardiac Myocytes
Alternative Antibody Names Anti-Alpha-1a Adrenergic Receptor antibody
Anti-Alpha-1a Adrenoreceptor antibody
Anti-Alpha-1a Adrenoceptor antibody
Anti-Alpha-1c Adrenergic Receptor antibody
Anti-Alpha-Adrenergic Receptor 1c antibody
Anti-ADRA1A antibody
Anti-ADRA1C antibody

Information sourced from Uniprot.org

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