| Host: | Mouse |
| Applications: | ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-ADIPOQ is suitable for use in ELISA research applications. |
| Clonality : | Monoclonal |
| Clone ID : | 1902 |
| Conjugation: | Unconjugated |
| Isotype: | IgG2b |
| Formulation: | 50 mM Na-citrate, pH 6.0, 0.9 % NaCI, 0.095 % NaN3 as a preservative |
| Purification: | Purity accurate to greater than or equal to 95% |
| Concentration: | 5.0 mg/ml (+/-10 %) |
| Storage Instruction: | 12 months from manufacturing at 2°C. Temperature analysis:-70 °C, 21 days N/D-20 °C, 21 days OK +4 °C, 21 days OK +30 °C, 21 OK +35 °C, 21 days OK +45 °C, 7 days OK. Shelf life stability testing at 2–8 °C in the product buffer. |
| Association Rate Constant: | 8.9 x 105 1/Ms |
| Determination Method: | SPR analysis (ProteOn XPR36) |
| Immunoreactivity: | 80–120 % compared to the reference sample in an FIA test |
| Gene Symbol: | ADIPOQ |
| Gene ID: | 9370 |
| Uniprot ID: | ADIPO_HUMAN |
| Immunogen: | human adiponectin |
| Tissue Specificity | Synthesized exclusively by adipocytes and secreted into plasma. |
| Post Translational Modifications | HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagen-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes. O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation. Succination of Cys-36 by the Krebs cycle intermediate fumarate, which leads to S-(2-succinyl)cysteine residues, inhibits polymerization and secretion of adiponectin. Adiponectin is a major target for succination in both adipocytes and adipose tissue of diabetic mammals. It was proposed that succination of proteins is a biomarker of mitochondrial stress and accumulation of Krebs cycle intermediates in adipose tissue in diabetes and that succination of adiponectin may contribute to the decrease in plasma adiponectin in diabetes. |
| Function | Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. |
| Protein Name | Adiponectin 30 Kda Adipocyte Complement-Related Protein Adipocyte Complement-Related 30 Kda Protein Acrp30 Adipocyte - C1q And Collagen Domain-Containing Protein Adipose Most Abundant Gene Transcript 1 Protein Apm-1 Gelatin-Binding Protein |
| Database Links | Reactome: R-HSA-163680 Reactome: R-HSA-381340 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Adiponectin antibody Anti-30 Kda Adipocyte Complement-Related Protein antibody Anti-Adipocyte Complement-Related 30 Kda Protein antibody Anti-Acrp30 antibody Anti-Adipocyte - C1q And Collagen Domain-Containing Protein antibody Anti-Adipose Most Abundant Gene Transcript 1 Protein antibody Anti-Apm-1 antibody Anti-Gelatin-Binding Protein antibody Anti-ADIPOQ antibody Anti-ACDC antibody Anti-ACRP30 antibody Anti-APM1 antibody Anti-GBP28 antibody |
Information sourced from Uniprot.org