| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ADGRF5 |
| Gene ID: |
221395 |
| Uniprot ID: |
AGRF5_HUMAN |
| Immunogen Region: |
22-280 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 22-280 of human ADGRF5 (NP_056049.4). |
| Immunogen Sequence: |
ALNWNYESTIHPLSLHEHEP AGEEALRQKRAVATKSPTAE EYTVNIEISFENASFLDPIK AYLNSLSFPIHGNNTDQITD ILSINVTTVCRPAGNEIWCS CETGYGWPRERCLHNLICQE RDVFLPGHHCSCLKELPPNG PFCLLQEDVTLNMRVRLNVG FQEDLMNTSSALYRSYKTDL ETAFRKGYGILPGFKGVTVT GFKSGSVVVTYEVKTTPPSL ELIHKANEQVVQSLNQTYK |
| Tissue Specificity | Expressed in lung endothelial cells and in alveolar type II (ATII) cells (at protein level). Expressed high levels in subcutaneous adipose tissue in lean individuals and at lower levels in visceral fat. Expression levels in subcutaneous adipose tissue drastically drop in obese individuals. |
| Post Translational Modifications | Highly glycosylated. Proteolytically cleaved at multiple sites: one in the GPS region of the GAIN-B domain (S1 site) and the other in the SEA domain (S2 site). The proteolytic cleavage at S1 site generates an extracellular subunit and a seven-transmembrane subunit. The proteolytic cleavage at S2 site generates a fragment that undergoes proteolytic cleavage by the processing enzyme furin. |
| Function | Adhesion G protein-coupled receptor. In alveolar type II (ATII or AT2) cells, required for normal lung surfactant homeostasis. Modulation of both surfactant secretion and uptake by ATII cells is mediated by the downstream activation of GNAQ/GNA11 proteins and may be a consequence of increased cortical F-actin assembly induced by ADGRF5 activation. In the kidney, may play a role in the regulation of acid excretion into the primary urine, possibly by regulating the surface expression of V-ATPase proton pump. As a receptor for soluble FNDC4 (sFNDC4), required for proper systemic glucose tolerance, specifically sensitizing white adipose tissue to insulin. Plays also a role in sFNDC4-induced decrease of local inflammation in white adipose tissue. |
| Protein Name | Adhesion G Protein-Coupled Receptor F5G-Protein Coupled Receptor 116 |
| Database Links | Reactome: R-HSA-5683826 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Adhesion G Protein-Coupled Receptor F5 antibodyAnti-G-Protein Coupled Receptor 116 antibodyAnti-ADGRF5 antibodyAnti-GPR116 antibodyAnti-KIAA0758 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
