• Western blot analysis of extracts of various cell lines, using ADGRF5 antibody (STJ27181) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ADGRF5 antibody (22-280) (STJ27181)

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STJ27181

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ADGRF5 (22-280) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADGRF5
Gene ID: 221395
Uniprot ID: AGRF5_HUMAN
Immunogen Region: 22-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 22-280 of human ADGRF5 (NP_056049.4).
Immunogen Sequence: ALNWNYESTIHPLSLHEHEP AGEEALRQKRAVATKSPTAE EYTVNIEISFENASFLDPIK AYLNSLSFPIHGNNTDQITD ILSINVTTVCRPAGNEIWCS CETGYGWPRERCLHNLICQE RDVFLPGHHCSCLKELPPNG PFCLLQEDVTLNMRVRLNVG FQEDLMNTSSALYRSYKTDL ETAFRKGYGILPGFKGVTVT GFKSGSVVVTYEVKTTPPSL ELIHKANEQVVQSLNQTYK
Post Translational Modifications Proteolytically cleaved at multiple sites: one in the GPS domain (S1 site) and the other in the SEA domain (S2 site). The proteolytic cleavage at S1 site generates an extracellular subunit and a seven-transmembrane subunit. The proteolytic cleavage at S2 site generates a fragment that undergoes proteolytic cleavage by the processing enzyme furin. Highly glycosylated.
Function Receptor that plays a critical role in lung surfactant homeostasis. May play a role in controlling adipocyte function.
Protein Name Adhesion G Protein-Coupled Receptor F5
G-Protein Coupled Receptor 116
Database Links Reactome: R-HSA-5683826
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Adhesion G Protein-Coupled Receptor F5 antibody
Anti-G-Protein Coupled Receptor 116 antibody
Anti-ADGRF5 antibody
Anti-GPR116 antibody
Anti-KIAA0758 antibody

Information sourced from Uniprot.org

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