• Western blot analysis of extracts of various cell lines, using ADD1 antibody (STJ22515) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-ADD1 antibody (60-300) (STJ22515)

SKU:
STJ22515

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ADD1 (60-300) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADD1
Gene ID: 118
Uniprot ID: ADDA_HUMAN
Immunogen Region: 60-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 60-300 of human ADD1 (NP_054908.2).
Immunogen Sequence: MILQSPAFCEELESMIQEQF KKGKNPTGLLALQQIADFMT TNVPNVYPAAPQGGMAALNM SLGMVTPVNDLRGSDSIAYD KGEKLLRCKLAAFYRLADLF GWSQLIYNHITTRVNSEQEH FLIVPFGLLYSEVTASSLVK INLQGDIVDRGSTNLGVNQA GFTLHSAIYAARPDVKCVVH IHTPAGAAVSAMKCGLLPIS PEALSLGEVAYHDYHGILVD EEEKVLIQKNLGPKSKVLI
Tissue Specificity Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
Function Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
Protein Name Alpha-Adducin
Erythrocyte Adducin Subunit Alpha
Database Links Reactome: R-HSA-264870
Reactome: R-HSA-381038
Reactome: R-HSA-5223345
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Alternative Antibody Names Anti-Alpha-Adducin antibody
Anti-Erythrocyte Adducin Subunit Alpha antibody
Anti-ADD1 antibody
Anti-ADDA antibody

Information sourced from Uniprot.org

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