• Western blot analysis of lysates from wild type (WT) and Adenosine Deaminase (Adenosine Deaminase (ADA) ) knockout (KO) HeLa cells, using [KO Validated] Adenosine Deaminase (ADA) Rabbit polyclonal antibody (STJ11100002) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ADA antibody (1-363) (STJ11100002)

SKU:
STJ11100002

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ADA (1-363) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADA
Gene ID: 100
Uniprot ID: ADA_HUMAN
Immunogen Region: 1-363
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-363 of human Adenosine Deaminase (Adenosine Deaminase (ADA) ) (NP_000013.2).
Immunogen Sequence: MAQTPAFDKPKVELHVHLDG SIKPETILYYGRRRGIALPA NTAEGLLNVIGMDKPLTLPD FLAKFDYYMPAIAGCREAIK RIAYEFVEMKAKEGVVYVEV RYSPHLLANSKVEPIPWNQA EGDLTPDEVVALVGQGLQEG ERDFGVKARSILCCMRHQPN WSPKVVELCKKYQQQTVVAI DLAGDETIPGSSLLPGHVQA YQEAVKSGIHRTVHAGEVGS AEVVKEAVDILKTERLGHG
Tissue Specificity Found in all tissues, occurs in large amounts in T-lymphocytes. Expressed at the time of weaning in gastrointestinal tissues.
Function Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. Enhances dendritic cell immunogenicity by affecting dendritic cell costimulatory molecule expression and cytokines and chemokines secretion. Enhances CD4+ T-cell differentiation and proliferation. Acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A, by enhancing their ligand affinity via conformational change. Stimulates plasminogen activation. Plays a role in male fertility. Plays a protective role in early postimplantation embryonic development.
Protein Name Adenosine Deaminase
Adenosine Aminohydrolase
Database Links Reactome: R-HSA-74217
Reactome: R-HSA-9734735
Reactome: R-HSA-9755088
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Extracellular Side
Cell Junction
Cytoplasmic Vesicle Lumen
Cytoplasm
Lysosome
Colocalized With Dpp4 At The Cell Surface
Alternative Antibody Names Anti-Adenosine Deaminase antibody
Anti-Adenosine Aminohydrolase antibody
Anti-ADA antibody
Anti-ADA1 antibody

Information sourced from Uniprot.org

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