• Western blot analysis of lysate from L929 cells, using eIF5A (Acetyl-Lys47) Antibody.
  • Western blot analysis of L929 lysis using antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-Acetyl-EIF5A2-K47 antibody (11-60) (STJ90136)


Current Stock:
Host: Rabbit
Applications: WB, ELISA
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal antibody anti-Acetyl-Eukaryotic Translation Initiation Factor 5a-2-K47 (11-60) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit anti-serum by affinity-chromatography.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF5A2
Gene ID: 56648
Uniprot ID: IF5A2_HUMAN
Immunogen Region: 11-60
Specificity: Acetyl-EIF5A2-K47 polyclonal antibody (Eukaryotic Translation Initiation Factor 5a-2) binds to endogenous Eukaryotic Translation Initiation Factor 5a-2 at the amino acid region 11-60.
Immunogen: The antiserum was produced against synthesized Acetyl-peptide derived from human eIF5A around the Acetylation site of Lys47 at amino acid range 11-60
Post Translational Modifications eIF-5A seems to be the only eukaryotic protein to have a hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).
Function mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation.
Protein Name Eukaryotic Translation Initiation Factor 5a-2
Eukaryotic Initiation Factor 5a Isoform 2
Database Links Reactome: R-HSA-204626
Cellular Localisation Cytoplasm
Endoplasmic Reticulum Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Nuclear Pore Complex
Hypusine Modification Promotes The Nuclear Export And Cytoplasmic Localization And There Was A Dynamic Shift In The Localization From Predominantly Cytoplasmic To Primarily Nuclear Under Apoptotic Inducing Conditions
Alternative Antibody Names Anti-Eukaryotic Translation Initiation Factor 5a-2 antibody
Anti-Eif-5a-2 antibody
Anti-Eif-5a2 antibody
Anti-Eukaryotic Initiation Factor 5a Isoform 2 antibody
Anti-EIF5A2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance