• Western blot analysis of extracts of HL-60 cells, using ABCG5 antibody (STJ111321) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ABCG5 antibody (350-450) (STJ111321)


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Host: Rabbit
Applications: WB
Reactivity: Human
Short Description: Rabbit polyclonal antibody anti-ABCG5 (350-450) is suitable for use in Western Blot.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS containing 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Gene Symbol: ABCG5
Gene ID: 64240
Immunogen Region: 350-450
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 350-450 of human ABCG5 (NP_071881.1).
Tissue Specificity Strongly expressed in the liver, lower levels in the small intestine and colon.
Post Translational Modifications N-glycosylated.
Function ABCG5 and ABCG8 form an obligate heterodimer that mediates Mg(2+)- and ATP-dependent sterol transport across the cell membrane. Plays an essential role in the selective transport of dietary plant sterols and cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. Required for normal sterol homeostasis. The heterodimer with ABCG8 has ATPase activity.
Protein Name Atp-Binding Cassette Sub-Family G Member 5
Database Links Reactome: R-HSA-1369062
Reactome: R-HSA-5679090
Reactome: R-HSA-5679096
Reactome: R-HSA-9029569
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Apical Cell Membrane
Alternative Antibody Names Anti-Atp-Binding Cassette Sub-Family G Member 5 antibody
Anti-Sterolin-1 antibody
Anti-ABCG5 antibody

Information sourced from Uniprot.org

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