• Western blot analysis of extracts of various cell lines, using AARS rabbit polyclonal antibody (STJ117211) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry of paraffin-embedded rat spleen using AARS antibody (STJ117211) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human lung cancer using AARS antibody (STJ117211) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse liver using AARS antibody (STJ117211) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of 293T cells using AARS antibody (STJ117211) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-AARS antibody (1-100) (STJ117211)

SKU:
STJ117211

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Host: Rabbit
Applications: WB, IHC, IF
Reactivity: Human, Mouse, Rat
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody anti-AARS (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS containing 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:50-1:200
IF 1:50-1:200
Storage Instruction: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Gene Symbol: AARS1
Gene ID: 16
Uniprot ID: SYAC_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human AARS (NP_001596.2).
Post Translational Modifications ISGylated.
Function Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.
Protein Name Alanine--Trna Ligase - Cytoplasmic
Alanyl-Trna Synthetase
Alars
Renal Carcinoma Antigen Ny-Ren-42
Database Links Reactome: R-HSA-379716
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Alanine--Trna Ligase - Cytoplasmic antibody
Anti-Alanyl-Trna Synthetase antibody
Anti-Alars antibody
Anti-Renal Carcinoma Antigen Ny-Ren-42 antibody
Anti-AARS1 antibody
Anti-AARS antibody

Information sourced from Uniprot.org

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