• Western blot analysis of extracts of various cell lines, using A-RAF antibody (STJ11103628) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry of paraffin-embedded human liver using A-RAF rabbit monoclonal antibody (STJ11103628) at dilution of 1:100 (40x lens).

Anti-A-RAF antibody (STJ11103628)

Current Stock:
Host: Rabbit
Applications: WB, IHC
Reactivity: Human
Short Description: Rabbit monoclonal antibody anti-A-RAF is suitable for use in Western Blot and Immunohistochemistry.
Clonality: Monoclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS containing 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:50-1:200
Storage Instruction: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Gene Symbol: ARAF
Gene ID: 369
Immunogen: A synthesized peptide derived from human A-RAF
Tissue Specificity Predominantly in urogenital tissues.
Function Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade. Isoform 2: Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation.
Protein Name Serine/Threonine-Protein Kinase A-Raf
Proto-Oncogene A-Raf
Proto-Oncogene A-Raf-1
Proto-Oncogene Pks
Database Links Reactome: R-HSA-5673000
Reactome: R-HSA-5674135
Reactome: R-HSA-5675221
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-9649948
Reactome: R-HSA-9656223
Reactome: R-HSA-9660537
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase A-Raf antibody
Anti-Proto-Oncogene A-Raf antibody
Anti-Proto-Oncogene A-Raf-1 antibody
Anti-Proto-Oncogene Pks antibody
Anti-ARAF antibody
Anti-ARAF1 antibody
Anti-PKS antibody
Anti-PKS2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance