• Western blot analysis of lysates from HeLa cells, using 4-HNE Rabbit polyclonal antibody (STJ11105326) at 1:1000 dilution. HeLa treated with 4-Hydroxynonenal (4-HNE). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-4-HNE antibody (STJ11105326)

SKU:
STJ11105326

£116.50 - £543.50
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Species independent
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-4-HNE is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthesized peptide derived from 4-HNE.
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance