When to use Flow Cytometry?
Loading Control Antibody Guide
Knockout-validated primary antibodies
Secondary antibodies resources
Alexa Fluor secondary antibodies
Biotinylated secondary antibodies
Gold-labelled secondary antibodies
How to prepare Phosphate Buffered Saline (PBS)
How to design a flow cytometry panel
Enhancing Detection of Low-Abundance Proteins
9 tips for detecting phosphorylation events using a Western Blot
Western Blotting with Tissue Lysates
Western blot membrane stripping protocol
Immunohistochemistry and Immunocytochemistry
Chromogenic and Fluorescent detection
Preparing paraffin-embedded and frozen samples for Immunohistochemistry
Competitive ELISA assay protocol
Measuring analyte concentration using serial dilutions and standard curve
When to use Flow Cytometry
What is immunophenotyping?
The urge to detect the elevated number of cell subsets in the immune system is the reason for multicolour flow cytometry to become the most common practice in identifying cell markers.
Immunophenotyping can be a simple cell identification by only a single marker or a complex procedure, involving homing profile, cytokine release and activation sites in a single panel. Apart from research, this technique is also quite useful in clinic diagnostics or routine monitoring.
What is apoptosis?
How is apoptosis measured?
How can cell proliferation be measured?
Using cytoplasmic dyes
Another way to measure proliferation is by using cytoplasmic dyes. After incubation with these protein-binding dyes, cell division becomes more visible. This is because when the cell divides, the concentration in the new cells is halved, allowing us to observe the reduced fluorescence levels in the following generations.