• VD (Vitamin D) Competitive ELISA (STJE0006574)

VD (Vitamin D) Competitive ELISA (STJE0006574)

SKU:
STJE0006574

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Applications: ELISA
Reactivity: All
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 3.75ng/mL
Detection Limit: 6.25~400ng/mL
Short Description: This VD Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in all cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 2.5h
Detection: Colormetric
Specificity: This kit recognizes VD in samples. No significant cross-reactivity or interference between VD and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Specificity This kit recognizes VD in samples. No significant cross-reactivity or interference between VD and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 96-112 102
EDTA plasma(n=8) 88-99 93
Cell culture media(n=8) 85-97 91

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 21.62 53.66 157.91 21.41 57.30 152.81
Standard deviation 1.28 2.70 5.98 1.47 2.35 8.34
CV (%) 5.92 5.03 3.79 6.87 4.10 5.46
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance