| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
TMEM16A Positive Control for STJ503327 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: |
WB: 1:500 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
ANO1 |
| Gene ID: |
55107 |
| Uniprot ID: |
ANO1_HUMAN |
| Specificity: |
This is positive control is recommended for use in combination with TMEM16A antibody STJ503327. |
| Tissue Specificity | Expressed in nasal epithelial cells (at protein level). In the kidney, expressed in the collecting duct (at protein level). Broadly expressed with higher levels in liver, skeletal muscle and gastrointestinal muscles. Expressed in eccrine sweat glands. |
| Function | Calcium-activated chloride channel (CaCC). Plays a role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development. Required for CFTR activation by enhancing endoplasmic reticulum Ca(2+) store release and is also required for CFTR membrane expression. Required for basal and ATP-dependent mucus secretion in airways and intestine, probably by controlling exocytosis of mucus-filled granules by providing Ca(2+) to an apical signaling compartment. Contributes to airway mucus expression induced by interleukins IL3 and IL8 and by the asthma-associated protein CLCA1 and is required for expression of mucin MUC5AC. However, was shown in another study not to be required for MUC5AC expression. Plays a role in the propagation of Ca(2+) waves in Kolliker's organ in the cochlea and contributes to the refinement of auditory brainstem circuitries prior to hearing onset. In vomeronasal sensory neurons, modulates spontaneous firing patterns in the absence of stimuli as well as the firing pattern of pheromone-evoked activity. Responsible for calcium-activated chloride channel activity in type I taste cells of the vallate papillae. Acts as a heat sensor in nociceptive neurons. In dorsal root ganglion neurons, plays a role in mediating non-histaminergic Mas-related G-protein coupled receptor (MRGPR)-dependent itching, acting as a downstream effector of MRGPRs. In the developing brain, required for the Ca(2+)-dependent process extension of radial glial cells. Isoform 4: Calcium-activated chloride channel (CaCC). Contributes to calcium-activated chloride secretion in human sweat gland epithelial cells. Shows increased basal chloride permeability and decreased Ca(2+)-induced chloride permeability. Isoform 5: Calcium-activated chloride channel (CaCC). Shows increased sensitivity to intracellular Ca(2+). |
| Peptide Name | Anoctamin-1Discovered On Gastrointestinal Stromal Tumors Protein 1Oral Cancer Overexpressed Protein 2Transmembrane Protein 16aTumor-Amplified And Overexpressed Sequence 2 |
| Database Links | Reactome: R-HSA-2672351Reactome: R-HSA-9733458 |
| Cellular Localisation | Apical Cell MembraneMulti-Pass Membrane ProteinPresynapseIn Differentiating Airway Epithelial CellsPredominantly Intracellular At Day 0 But Is Apically Localized By Day 30Expressed In The Presynapse Of Retinal Neurons |
| Alternative Peptide Names | Anoctamin-1 proteinDiscovered On Gastrointestinal Stromal Tumors Protein 1 proteinOral Cancer Overexpressed Protein 2 proteinTransmembrane Protein 16a proteinTumor-Amplified And Overexpressed Sequence 2 proteinANO1 proteinDOG1 proteinORAOV2 proteinTAOS2 proteinTMEM16A protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
