| Applications: | Immunodepletion/Immunocompetition |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | RUNX3 Blocking Peptide for STJ502845 is synthetically produced from the 200-250 sequence and is suitable for use in western blot applications. |
| Formulation: | Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice. |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | RUNX3 |
| Gene ID: | 864 |
| Uniprot ID: | RUNX3_HUMAN |
| Immunogen Region: | 200-250 |
| Specificity: | This blocking peptide is recommended for use in combination with RUNX3 antibody, STJ502845 |
| Immunogen: | Synthetic peptide taken within amino acid region 200-250 on human Runt-related transcription factor 3 protein. |
| Tissue Specificity | Expressed in gastric cancer tissues (at protein level). |
| Post Translational Modifications | Phosphorylated on tyrosine residues by SRC. Phosphorylated by LCK and FYN. |
| Function | Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. May be involved in the control of cellular proliferation and/or differentiation. In association with ZFHX3, up-regulates CDKN1A promoter activity following TGF-beta stimulation. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing. |
| Peptide Name | Runt-Related Transcription Factor 3 Acute Myeloid Leukemia 2 Protein Core-Binding Factor Subunit Alpha-3 Cbf-Alpha-3 Oncogene Aml-2 Polyomavirus Enhancer-Binding Protein 2 Alpha C Subunit Pea2-Alpha C Pebp2-Alpha C Sl3-3 Enhancer Factor 1 Alpha C Subunit Sl3/Akv Core-Binding Factor Alpha C Subunit |
| Database Links | Reactome: R-HSA-4411364 Reactome: R-HSA-8941855 Reactome: R-HSA-8941856 Reactome: R-HSA-8941858 Reactome: R-HSA-8949275 Reactome: R-HSA-8951430 Reactome: R-HSA-8951671 Reactome: R-HSA-8951911 Reactome: R-HSA-8951936 Reactome: R-HSA-8952158 |
| Cellular Localisation | Nucleus Cytoplasm The Tyrosine Phosphorylated Form Localizes To The Cytoplasm Translocates From The Cytoplasm To The Nucleus Following Tgf-Beta Stimulation |
| Alternative Peptide Names | Runt-Related Transcription Factor 3 protein Acute Myeloid Leukemia 2 Protein protein Core-Binding Factor Subunit Alpha-3 protein Cbf-Alpha-3 protein Oncogene Aml-2 protein Polyomavirus Enhancer-Binding Protein 2 Alpha C Subunit protein Pea2-Alpha C protein Pebp2-Alpha C protein Sl3-3 Enhancer Factor 1 Alpha C Subunit protein Sl3/Akv Core-Binding Factor Alpha C Subunit protein RUNX3 protein AML2 protein CBFA3 protein PEBP2A3 protein |
Information sourced from Uniprot.org