| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This Vegfa Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Vegfa |
| Gene ID: |
83785 |
| Uniprot ID: |
VEGFA_RAT |
| Specificity: |
This kit recognizes Rat VEGF-A in samples. No significant cross-reactivity or interference between Rat VEGF-A and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. Also binds the DEAR/FBXW7-AS1 receptor. |
| Protein Name | Vascular Endothelial Growth Factor AVegf-AVascular Permeability FactorVpf |
| Database Links | Reactome: R-RNO-114608Reactome: -RNO-194313Reactome: -RNO-195399Reactome: -RNO-4420097Reactome: -RNO-5218921 |
| Cellular Localisation | SecretedVegf-A120 Is Acidic And Freely SecretedVegf-A164 Is More BasicHas Heparin-Binding Properties AndAlthough A Significant Proportion Remains Cell-AssociatedMost Is Freely SecretedVegf-A188 Is Very BasicIt Is Cell-Associated After Secretion And Is Bound Avidly By Heparin And The Extracellular MatrixAlthough It May Be Released As A Soluble Form By HeparinHeparinase Or Plasmin |
| Alternative ELISA Names | Vascular Endothelial Growth Factor A ELISA kitVegf-A ELISA kitVascular Permeability Factor ELISA kitVpf ELISA kitVegfa ELISA kitVegf ELISA kit |
| Specificity | This kit recognizes Rat VEGF-A in samples. No significant cross-reactivity or interference between Rat VEGF-A and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-101 | 93 |
| EDTA plasma (n=8) | 90-102 | 95 |
| Cell culture media (n=8) | 89-101 | 95 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 97.90 | 170.39 | 948.99 | 89.62 | 158.20 | 968.59 |
| Standard deviation | 5.02 | 9.25 | 46.60 | 5.65 | 7.26 | 37.97 |
| CV (%) | 5.13 | 5.43 | 4.91 | 6.30 | 4.59 | 3.92 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
