| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This rat tPA kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Plat |
| Gene ID: |
25692 |
| Uniprot ID: |
TPA_RAT |
| Specificity: |
This kit recognizes Rat tPA in samples. No significant cross-reactivity or interference between Rat tPA and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-308 catalyzed by plasmin, tissue kallikrein or factor Xa. |
| Function | Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. During oocyte activation, plays a role in cortical granule reaction in the zona reaction, which contributes to the block to polyspermy. |
| Protein Name | Tissue-Type Plasminogen ActivatorT-PaT-Plasminogen ActivatorTpa Cleaved Into - Tissue-Type Plasminogen Activator Chain A - Tissue-Type Plasminogen Activator Chain B |
| Database Links | Reactome: R-RNO-186797Reactome: -RNO-75205 |
| Cellular Localisation | SecretedExtracellular Space |
| Alternative CLIA Names | Tissue-Type Plasminogen Activator CLIA kitT-Pa CLIA kitT-Plasminogen Activator CLIA kitTpa Cleaved Into - Tissue-Type Plasminogen Activator Chain A - Tissue-Type Plasminogen Activator Chain B CLIA kitPlat CLIA kit |
| Specificity | This kit recognizes Rat tPA in samples. No significant cross-reactivity or interference between Rat tPA and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 90-107 | 98 |
| EDTA plasma(n=8) | 87-100 | 92 |
| Cell culture media(n=8) | 89-101 | 96 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 219.85 | 595.24 | 1904.91 | 210.82 | 584.31 | 1848.28 |
| Standard deviation | 20.47 | 50.30 | 151.06 | 16.91 | 62.05 | 124.20 |
| CV (%) | 9.31 | 8.45 | 7.93 | 8.02 | 10.62 | 6.72 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
