| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This Timp1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Timp1 |
| Gene ID: |
116510 |
| Uniprot ID: |
TIMP1_RAT |
| Specificity: |
This kit recognizes Rat TIMP-1 in samples. No significant cross-reactivity or interference between Rat TIMP-1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Also stimulates steroidogenesis by Leydig and ovarian granuloma cells.procathepsin L is required for maximal activity. |
| Protein Name | Metalloproteinase Inhibitor 1Tissue Inhibitor Of Metalloproteinases 1Timp-1 |
| Database Links | Reactome: R-RNO-114608Reactome: -RNO-1592389Reactome: -RNO-381426Reactome: -RNO-8957275 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Metalloproteinase Inhibitor 1 ELISA kitTissue Inhibitor Of Metalloproteinases 1 ELISA kitTimp-1 ELISA kitTimp1 ELISA kitTimp-1 ELISA kit |
| Specificity | This kit recognizes Rat TIMP-1 in samples. No significant cross-reactivity or interference between Rat TIMP-1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 92-107 | 99 |
| EDTA plasma (n=8) | 87-99 | 93 |
| Cell culture media (n=8) | 90-103 | 97 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.50 | 1.60 | 3.90 | 0.50 | 1.70 | 3.80 |
| Standard deviation | 0.03 | 0.08 | 0.14 | 0.03 | 0.10 | 0.20 |
| CV (%) | 6.00 | 5.00 | 3.59 | 6.00 | 5.88 | 5.26 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
