• Rat TFF2 (Trefoil Factor 2) CLIA Kit (STJC0001926)

Rat TFF2 (Trefoil Factor 2) CLIA Kit (STJC0001926)

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STJC0001926

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Applications: CLIA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This rat TFF2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: Tff2
Gene ID: 116592
Uniprot ID: TFF2_RAT
Specificity: This kit recognizes Rat TFF2 in samples. No significant cross-reactivity or interference between Rat TFF2 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Expressed in the digestive tract, where it was found predominantly in the stomach with highest expression in the antrum. It is secreted predominantly from antral mucous cells into the lumen of the gastrointestinal tract.
Function Inhibits gastrointestinal motility and gastric acid secretion. Could function as a structural component of gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains.
Protein Name Trefoil Factor 2
Spasmolytic Polypeptide
Sp
Cellular Localisation Secreted
Alternative CLIA Names Trefoil Factor 2 CLIA kit
Spasmolytic Polypeptide CLIA kit
Sp CLIA kit
Tff2 CLIA kit
Sml1 CLIA kit
Specificity This kit recognizes Rat TFF 2 in samples. No significant cross-reactivity or interference between Rat TFF 2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 84-96 91
EDTA plasma(n=8) 99-110 105
Cell culture media(n=8) 93-110 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 47.71 150.17 369.85 45.84 148.62 348.08
Standard deviation 5.27 12.73 31.36 5.50 16.66 29.83
CV (%) 11.05 8.48 8.48 12.00 11.21 8.57
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance