| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This rat TFF2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Tff2 |
| Gene ID: |
116592 |
| Uniprot ID: |
TFF2_RAT |
| Specificity: |
This kit recognizes Rat TFF2 in samples. No significant cross-reactivity or interference between Rat TFF2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in the digestive tract, where it was found predominantly in the stomach with highest expression in the antrum. It is secreted predominantly from antral mucous cells into the lumen of the gastrointestinal tract. |
| Function | Inhibits gastrointestinal motility and gastric acid secretion. Could function as a structural component of gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains. |
| Protein Name | Trefoil Factor 2Spasmolytic PolypeptideSp |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Trefoil Factor 2 CLIA kitSpasmolytic Polypeptide CLIA kitSp CLIA kitTff2 CLIA kitSml1 CLIA kit |
| Specificity | This kit recognizes Rat TFF 2 in samples. No significant cross-reactivity or interference between Rat TFF 2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 84-96 | 91 |
| EDTA plasma(n=8) | 99-110 | 105 |
| Cell culture media(n=8) | 93-110 | 100 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 47.71 | 150.17 | 369.85 | 45.84 | 148.62 | 348.08 |
| Standard deviation | 5.27 | 12.73 | 31.36 | 5.50 | 16.66 | 29.83 |
| CV (%) | 11.05 | 8.48 | 8.48 | 12.00 | 11.21 | 8.57 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
