| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This rat SELP kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Selp |
| Gene ID: |
25651 |
| Uniprot ID: |
LYAM3_RAT |
| Specificity: |
This kit recognizes Rat SELP in samples. No significant cross-reactivity or interference between Rat SELP and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Not detected in the absence of exposure to lipopolysaccharide (LPS). Detected only after exposure to lipopolysaccharide (LPS) in the tissues examined: spleen, lung, brain, liver, heart, kidney, thymus and small intestine. |
| Function | Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG. |
| Protein Name | P-SelectinCd62 Antigen-Like Family Member PGranule Membrane Protein 140Gmp-140Leukocyte-Endothelial Cell Adhesion Molecule 3Lecam3Platelet Activation Dependent Granule-External Membrane ProteinPadgemCd Antigen Cd62p |
| Database Links | Reactome: R-RNO-114608Reactome: -RNO-202733 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative CLIA Names | P-Selectin CLIA kitCd62 Antigen-Like Family Member P CLIA kitGranule Membrane Protein 140 CLIA kitGmp-140 CLIA kitLeukocyte-Endothelial Cell Adhesion Molecule 3 CLIA kitLecam3 CLIA kitPlatelet Activation Dependent Granule-External Membrane Protein CLIA kitPadgem CLIA kitCd Antigen Cd62p CLIA kitSelp CLIA kit |
| Specificity | This kit recognizes Rat SELP in samples. No significant cross-reactivity or interference between Rat SELP and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-98 | 91 |
| EDTA plasma(n=8) | 87-102 | 94 |
| Cell culture media(n=8) | 85-99 | 91 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 214.27 | 528.18 | 1716.48 | 219.92 | 502.60 | 1571.84 |
| Standard deviation | 17.63 | 37.45 | 130.97 | 18.36 | 58.20 | 145.24 |
| CV (%) | 8.23 | 7.09 | 7.63 | 8.35 | 11.58 | 9.24 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
