| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This rat RTN4 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Rtn4 |
| Gene ID: |
83765 |
| Uniprot ID: |
RTN4_RAT |
| Specificity: |
This kit recognizes Rat RTN4 in samples. No significant cross-reactivity or interference between Rat RTN4 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Isoforms A, B and C are present in optic nerve, spinal cord and cerebral cortex. Isoforms A and B are present in dorsal root ganglion, sciatic nerve and PC12 cells after longer exposure. Isoforms B and C are detected in kidney, cartilage, skin, lung and spleen. Isoform C is expressed at high level in skeletal muscle. In adult animals isoform A is expressed mainly in the nervous system. |
| Function | Required to induce the formation and stabilization of endoplasmic reticulum (ER) tubules. They regulate membrane morphogenesis in the ER by promoting tubular ER production. They influence nuclear envelope expansion, nuclear pore complex formation and proper localization of inner nuclear membrane proteins. However each isoform have specific functions mainly depending on their tissue expression specificities. Isoform A: Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex. Acts as a negative regulator of central nervous system angiogenesis. Inhibits spreading, migration and sprouting of primary brain microvascular endothelial cells (MVECs). Also induces the retraction of MVECs lamellipodia and filopodia in a ROCK pathway-dependent manner. Isoform B: Mainly function in endothelial cells and vascular smooth muscle cells, is also involved in immune system regulation. Modulator of vascular remodeling, promotes the migration of endothelial cells but inhibits the migration of vascular smooth muscle cells. Regulates endothelial sphingolipid biosynthesis with direct effects on vascular function and blood pressure. Inhibits serine palmitoyltransferase, SPTLC1, the rate-limiting enzyme of the novo sphingolipid biosynthetic pathway, thereby controlling production of endothelial sphingosine-1-phosphate (S1P). Required to promote macrophage homing and functions such as cytokine/chemokine gene expression involved in angiogenesis, arteriogenesis and tissue repair. Mediates ICAM1 induced transendothelial migration of leukocytes such as monocytes and neutrophils and acute inflammation. Necessary for immune responses triggered by nucleic acid sensing TLRs, such as TLR9, is required for proper TLR9 location to endolysosomes. Also involved in immune response to LPS. Plays a role in liver regeneration through the modulation of hepatocytes proliferation. Reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. With isoform C, inhibits BACE1 activity and amyloid precursor protein processing. Isoform C: Regulates cardiomyocyte apoptosis upon hypoxic conditions. With isoform B, inhibits BACE1 activity and amyloid precursor protein processing. |
| Protein Name | Reticulon-4FoocenGlut4 Vesicle 20 Kda ProteinNeurite Outgrowth InhibitorNogo Protein |
| Database Links | Reactome: R-RNO-193634 |
| Cellular Localisation | Isoform A: Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinCell MembraneCytoplasmic SideAnchored To The Membrane Of The Endoplasmic Reticulum (Er) Through 2 Putative Transmembrane DomainsLocalizes Throughout The Er Tubular NetworkCo-Localizes With Tmem33 At The Er SheetsIsoform B: Endoplasmic Reticulum MembraneExtracellular SideCell JunctionMainly Located On Endoplasmic Reticulum Tubules And Sheet EdgesUpon Icam1 EngagementRedistributed Toward Endothelial Junctions Where Interacts With Cdh5Isoform C: Endoplasmic Reticulum Membrane |
| Alternative CLIA Names | Reticulon-4 CLIA kitFoocen CLIA kitGlut4 Vesicle 20 Kda Protein CLIA kitNeurite Outgrowth Inhibitor CLIA kitNogo Protein CLIA kitRtn4 CLIA kitNogo CLIA kit |
| Specificity | This kit recognizes Rat RTN4 in samples. No significant cross-reactivity or interference between Rat RTN4 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-104 | 95 |
| EDTA plasma(n=8) | 98-113 | 106 |
| Cell culture media(n=8) | 88-102 | 96 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 219.68 | 420.00 | 1974.59 | 205.26 | 396.76 | 1845.96 |
| Standard deviation | 23.90 | 36.25 | 152.04 | 18.14 | 34.64 | 163.00 |
| CV (%) | 10.88 | 8.63 | 7.70 | 8.84 | 8.73 | 8.83 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
