| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This rat PRF1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Prf1Uniprot ID=PERF_RAT" |
| Specificity: |
This kit recognizes Rat PRF1 in samples. No significant cross-reactivity or interference between Rat PRF1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Detected in large granular lymphocytes and lymphokine-activated killer cells. |
| Post Translational Modifications | N-glycosylated. |
| Function | Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes. Facilitates the delivery of cationic cargo protein, while anionic or neural proteins are not delivered efficiently. Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu. |
| Protein Name | Perforin-1P1CytolysinLymphocyte Pore-Forming Protein |
| Cellular Localisation | Cytolytic GranuleSecretedCell MembraneMulti-Pass Membrane ProteinEndosome LumenStored In Cytolytic Granules Of Cytolytic T-Lymphocytes And Secreted Into The Cleft Between T-Lymphocyte And Target CellInserts Into The Cell Membrane Of Target Cells And Forms PoresMembrane Insertion And Pore Formation Requires A Major Conformation ChangeMay Be Taken Up Via Endocytosis Involving Clathrin-Coated Vesicles And Accumulate In A First Time In Large Early Endosomes |
| Alternative CLIA Names | Perforin-1 CLIA kitP1 CLIA kitCytolysin CLIA kitLymphocyte Pore-Forming Protein CLIA kitPrf1 CLIA kitPfp CLIA kit |
| Specificity | This kit recognizes Rat PRF1 in samples. No significant cross-reactivity or interference between Rat PRF1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-104 | 95 |
| EDTA plasma(n=8) | 90-102 | 97 |
| Cell culture media(n=8) | 84-97 | 90 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 215.91 | 619.45 | 1357.54 | 209.82 | 567.98 | 1404.47 |
| Standard deviation | 22.80 | 58.79 | 95.30 | 25.60 | 64.64 | 109.55 |
| CV (%) | 10.56 | 9.49 | 7.02 | 12.20 | 11.38 | 7.80 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
