| Applications: |
ELISA |
| Reactivity: |
Rat/Porcine |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
1.00pg/mL |
| Detection Limit: |
1.56~100pg/mL |
| Short Description: |
This E2 Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat/porcine cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
2.5h |
| Detection: |
Colormetric |
| Specificity: |
This kit recognizes Rat/Porcine E2 in samples. No significant cross-reactivity or interference between Rat/Porcine E2 and analogues was observed. |
| Sample Type: |
Serum and plasma |
| Specificity | This kit recognizes E2 in samples. No significant cross-reactivity or interference between E2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are < 10%. |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8℃ |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 80-91 | 84 |
| EDTA plasma(n=8) | 82-93 | 87 |
| Urine(n=8) | | 95 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 40.33 | 90.22 | 200.31 | 41.25 | 92.32 | 201.59 |
| Standard deviation | 3.52 | 6.80 | 10.67 | 3.76 | 7.36 | 12.55 |
| CV (%) | 8.75 | 7.54 | 5.33 | 9.12 | 7.98 | 6.23 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
