• Rat PDK4 (Pyruvate Dehydrogenase Kinase Isozyme 4) Sandwich ELISA Kit Ready-To-Use (STJE0017768)

Rat PDK4 (Pyruvate Dehydrogenase Kinase Isozyme 4) Sandwich ELISA Kit Ready-To-Use (STJE0017768)

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STJE0017768

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Applications: ELISA
Reactivity: Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 33.2pg/mL
Detection Limit: 78.1-5000pg/mL
Short Description: This PDK4 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 3 hrs
Gene Symbol: Pdk4
Gene ID: 89813
Uniprot ID: PDK4_RAT
Immunogen Region: Ready-To-Use
Sample Type: serum, plasma, tissue homogenates or other biological fluids.
Tissue Specificity Ubiquitous.highest levels of expression in heart and skeletal muscle.
Post Translational Modifications
Function Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation. Plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, mediates cellular responses to glucose levels and to a high-fat diet. Regulates both fatty acid oxidation and de novo fatty acid biosynthesis. Plays a role in the generation of reactive oxygen species. Protects detached epithelial cells against anoikis. Plays a role in cell proliferation via its role in regulating carbohydrate and fatty acid metabolism.
Protein Name Pyruvate Dehydrogenase
Acetyl-Transferring Kinase Isozyme 4 - Mitochondrial
Pyruvate Dehydrogenase Kinase Isoform 4
Database Links Reactome: R-RNO-204174
Reactome: -RNO-5362517
Cellular Localisation Mitochondrion Matrix
Alternative ELISA Names Pyruvate Dehydrogenase ELISA kit
Acetyl-Transferring Kinase Isozyme 4 - Mitochondrial ELISA kit
Pyruvate Dehydrogenase Kinase Isoform 4 ELISA kit
Pdk4 ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance